As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). Aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored at these stations. At each of these stations, benthic macroinvertebrates (bmi) have been sampled regularly, in spring and autumn, since September 2014 and in accordance with standard NF T 90-333. The fauna inventories obtained after determination can thus be used to calculate biotic indices such as the IBG-DCE and the I2M2. In addition, samples were taken using artificial substrates immersed for 1 month, in order to focus on measuring the "dam effect". The protocol is described in: Piscart C., Moreteau, J.C., Beisel J.N. (2006). Monitoring changes in freshwater macroinvertebrate communities along a salinity gradient using artificial substrates. Environmental Monitoring and Assessment 116: 529-542. This layer gives the locations of these stations.

The spawning grounds of the sea lamprey Petromyzon marinus are located within the perimeter of the Sélune Observatory. As this species is migratory, its colonisation of the Sélune river and its tributaries will probably be influenced by the removal of the Sélune dams. The spawning grounds are characterised by a depression accompanied by a sediment ejection dome immediately downstream. The stones returned to the dome are lighter, making the nests easily visible and identifiable for up to several weeks after spawning.

As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). At these stations, aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. At each of these stations, benthic macroinvertebrates (bmi) have been sampled regularly, in spring and autumn, since September 2014 and in accordance with standard NF T 90-333. The fauna inventories obtained after determination can thus be used to calculate biotic indices such as the IBG-DCE and the I2M2. In addition, samples were taken using artificial substrates immersed for 1 month, in order to focus on measuring the ‘dam effect’. The protocol is described in: Piscart C., Moreteau, J.C., Beisel J.N. (2006). Monitoring changes in freshwater macroinvertebrate communities along a salinity gradient using artificial substrates. Environmental Monitoring and Assessment 116: 529-542. This layer gives the results obtained for the IBG-DCE and I2M2 biotic indices with the associated parameters (diversity, variety, etc.), as well as the number of samples taken (‘effort’ column). Finally, the layer contains the output from the ‘ODInvertebres’ diagnostic tool for benthic invertebrates, which aims to identify the most likely anthropogenic pressures causing changes in ecological quality (6 probability values for physico-chemical water quality and 6 others relating to hydromorphology or land use in the catchment area). Reference on ‘ODInvertebres’ : Mondy, C. P., & Usseglio-Polatera P. Using conditional tree forests and life history traits to assess specific risks of stream degradation under multiple pressure scenario. Science of the Total Environment 461 (2013): 750-760. The genus Baetis cited in the fauna lists refers to the genus ‘Baetis lato sensu’ according to the SANDRE nomenclature (Sandre code: 9794). Taxa presenting all of the clearly visible and characteristic morphological criteria enabling them to be accurately identified and in an irreproachable state of conservation are included in the Observatoire Sélune's reference collection. This can be consulted on request (see point of contact).

As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). At these stations, aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. 2 sampling campaigns (spring and autumn) for benthic macroinvertebrates (bmi) have been carried out since September 2014. During each campaign, 2 sampling protocols are implemented: - in accordance with standard NF T90-333, which enables the calculation of the IBG-DCE and I2M2 (‘SURBER’) - via artificial substrates immersed for 1 month (‘SUBART’), in order to focus on measuring the ‘effect of dams’. The protocol is described in: Piscart C., Moreteau, J.C., Beisel J.N. (2006). Monitoring changes in freshwater macroinvertebrate communities along a salinity gradient using artificial substrates. Environmental Monitoring and Assessment 116: 529-542. This layer details the benthic macroinvertebrate taxa and their abundance obtained after analysis (according to standard NF T90-388). These data are distributed by campaign and by sector. Taxa presenting all the morphological criteria clearly visible and characteristic allowing the exactitude of their determination and an irreproachable state of conservation are integrated into the reference collection of the Sélune Observatory. This collection can be consulted on request (see contact point). Taxon determination is based on version v17 of the TAXREF reference.